What did you test and what were your findings?
Write us a report grade 11 style 😜
- Purpose
- Hypothesis 2.5. Counter-Thesis (polar opposite of hypothesis so both are tested for)
- Materials(if any)
- Procedure
- Observations
- Conclusions
What did you test and what were your findings?
Write us a report grade 11 style 😜
Contramuffin AP Biology Mrs. Setters 1 December 2024 How cells respond to different doses of drugs Purpose: The purpose of this experiment is to see whether cells respond differently when exposed to different doses of the same drug. Hypothesis: The drugs will work the best at the highest dosage, but it won't work at the lowest dosage. Alternate hypothesis: All of the dosages work equally well. Materials: * Cells * 48-well plate * Cell media * Recording device * Micropipettes * Micropipette tips (a lot) * Serological pipers (a lot) * Serological piper pump * Cell culture hood * Drug (5 mg) * DMSO (5 mL) * PBS (5 ml) * Stimulus (500 uL) * PCR strip (2) Procedure: 1. Using the cell culture hood, put cells and cell media into a 48-well plate 2. Put the plate into a recording device and start recording 3. Dilute drugs to the correct concentrations with DMSO 4. Put the drugs and the stimulant and the PBS into PCR strips for easier usage later 5. Stop the recording and put drugs into the plate 6. Wait 30 minutes 7. Put stimulant or PBS into the plate 8. Resume recording 9. After several days, stop the recording Results: The cells died :'( Conclusions: Science is hard